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Re: Engineering Mechanics: Statics, 6th Edition, Meriam ....rar ((LINK))


Re: Engineering Mechanics: Statics, 6th Edition, Meriam ....rar

Category:Journals and magazines relating to engineering Category:English-language journals Category:American educational websites Category:Springer Science+Business Media academic journalsStructure-Based Evolution of a Cytochrome P450 System for the Production of Complex Oxidized Products. A series of intermediate metabolic products of the bioactive sesquiterpene kalihinol A, produced by the fungus Pestalotiopsis microspora, were discovered using a combination of deuterium-exchange mass spectrometry and biosynthesis studies. As predicted, the products were formed by oxidation of the aromatic ring structures within the cytochrome P450-catalyzed oxidation of the kalihinol A precursor. Synthetic analogues of the compounds were used to show that the oxidation of the aromatic rings does not occur at the cytochrome P450, but at the kalihinol A substrate itself. The use of a biosynthetic approach allowed a wide range of structural analogues to be prepared and used to show that a solvent-accessible cavity is present on the protein surface. This work highlights the application of structure-based sequence design to improve the oxidation efficiency of an enzyme and suggests that this approach should be more widely applicable.

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Re: Engineering Mechanics: Statics, 6th Edition, Meriam ....rar ((LINK))

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